NRAS Polyklonaler Antikörper

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• KD/KO Validated

NRAS Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(34)

Rezensionen (1)

Kat-Nr. : 10724-1-AP

Synonyme

HRAS1, EC:3.6.5.2, GTPase NRas, N ras, NRAS1

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  WB analysis using 10724-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10724-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10724-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10724-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10724-1-AP

  IP result of anti-NRAS (IP:10724-1-AP, 4ug; Detection:10724-1-AP 1:4000) with HEK-293 cells lysate 1720 ug.

• 

  IHC staining of human pancreas using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10724-1-AP (NRAS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10724-1-AP (NRAS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 10724-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NRAS antibody (10724-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 10724-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NRAS Polyclonal antibody (10724-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, C6-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 29 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

10724-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA NRAS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NRAS fusion protein Ag1081
• Vollständiger Name: neuroblastoma RAS viral (v-ras) oncogene homolog
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC005219
• Gene symbol: NRAS
• Gene ID (NCBI): 4893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML).

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16INK4a levels.; Authors - Xue Hao
human	WB	Gut; RNA helicase DDX5 enables STAT1 mRNA translation and interferon signalling in hepatitis B virus replicating hepatocytes.; Authors - Jiazeng Sun
human	WB	Nat Commun; Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma; Authors - Perla Pucci
human	IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
mouse,human	WB	Biomaterials; Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.; Authors - Yuanyuan Liu
human	WB	Clin Cancer Res; Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling.; Authors - Chen Liu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Brandon (Verified Customer) (11-27-2018)

Worked excellent on mouse tissue from inducible tumor model!

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Tumor Tissue