NRBF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NRBF2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 24858-1-AP

Synonyme

Nuclear receptor-binding factor 2, NRBF-2, NRBF 2, COPR2, COPR1

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  WB analysis using 24858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24858-1-AP (NRBF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24858-1-AP

  IP result of anti-NRBF2 (IP:24858-1-AP, 4ug; Detection:24858-1-AP 1:2000) with HEK-293 cells lysate 2640ug.

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  IHC staining of human liver using 24858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24858-1-AP (NRBF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 24858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24858-1-AP (NRBF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 24858-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRBF2 antibody (24858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

24858-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA NRBF2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NRBF2 fusion protein Ag21403
• Vollständiger Name: nuclear receptor binding factor 2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC001345
• Gene symbol: NRBF2
• Gene ID (NCBI): 29982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NRBF2 also termed as Nuclear receptor-binding factor 2or COPR is a 287 amino acid protein, which Interacts with PPARA, PPARD and PPARG. NRBF2 as transcription activator may modulate transcriptional activation by target nuclear receptors. NRBF2 is expressed in keratinocytes, liver and placenta. NRBF2, which exists as two isoforms due to alternative splicing, is localized to both the nucleus and the cytoplasm.

Publikationen

Species	Application	Title
human	WB	Autophagy; The mammalian actin elongation factor ENAH/MENA contributes to autophagosome formation via its actin regulatory function; Authors - Yueheng Li
mouse	IHC,IF	J Neuroinflammation; Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH.; Authors - Hanhai Zeng ; KO Validated
human,mouse	WB,IHC,IF,CoIP	iScience; NRBF2 regulates the chemoresistance of small cell lung cancer by interacting with the P62 protein in the autophagy process.; Authors - Weitao Shen ; KD Validated
mouse	WB	Lab Invest; Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.; Authors - Xiaosen Ouyang
human	WB	Nat Struct Mol Biol; Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging; Authors - Rui Guo
mouse	WB	Front Behav Neurosci; NRBF2 plays a crucial role in the acquisition process of learning and memory, independent of the Vps34 complex; Authors - Songfen Wu