NRBP2 Polyklonaler Antikörper

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• KD/KO Validated

NRBP2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 21549-1-AP

Synonyme

NRBP2, pp9320, TRG 16, TRG16

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  WB analysis of mouse kidney using 21549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21549-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21549-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 21549-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 21549-1-AP

  IP result of anti-NRBP2 (IP:21549-1-AP, 4ug; Detection:21549-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 21549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21549-1-AP (NRBP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 21549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21549-1-AP (NRBP2 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, A549-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, humanes Hodengewebe, Maushirngewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

21549-1-AP bindet in WB, IP, IHC, ELISA NRBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NRBP2 fusion protein Ag16215
• Vollständiger Name: nuclear receptor binding protein 2
• Berechnetes Molekulargewicht: 501 aa, 58 kDa
• Beobachtetes Molekulargewicht: 33 kDa, 58 kDa
• GenBank-Zugangsnummer: BC113873
• Gene symbol: NRBP2
• Gene ID (NCBI): 340371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.

Publikationen

Species	Application	Title
human	WB,IHC	Front Oncol; NRBP2 Functions as a Tumor Suppressor and Inhibits Epithelial-to-Mesenchymal Transition in Breast Cancer.; Authors - Zhiyu Li ; KD Validated
human	WB	Ann Transl Med; PSMD12 promotes glioma progression by upregulating the expression of Nrf2.; Authors - Zhongyong Wang
human,mouse	WB,IHC	Mol Cell Biochem; TRG16, targeted by miR-765, inhibits breast cancer stem cell-like properties via regulating the NF-κB pathway.; Authors - Feng Chi ; KD Validated
human	WB	Cancers (Basel); Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression.; Authors - Anqi Xiong