NRCAM Monoklonaler Antikörper

NRCAM Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E1C3

Kat-Nr. : CL488-68291

Synonyme

hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

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  IF Staining of HEK-293 using CL488-68291

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 NRCAM antibody (CL488-68291, Clone: 2E1C3 ) at dilution of 1:400.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68291 bindet in IF/ICC NRCAM und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NRCAM fusion protein Ag20558
• Vollständiger Name: neuronal cell adhesion molecule
• Berechnetes Molekulargewicht: 1304 aa, 144 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC098401
• Gene symbol: NRCAM
• Gene ID (NCBI): 4897
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).