NRF1/nuclear respiratory factor 1 Monoklonaler Antikörper

NRF1/nuclear respiratory factor 1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C2B8

Kat-Nr. : CL488-66832

Synonyme

ALPHA PAL, NRF 1, NRF1, nuclear respiratory factor 1

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL488-66832

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NRF1 antibody (CL488-66832, Clone: 3C2B8 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-66832

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NRF1/nuclear respiratory factor 1 (CL488-66832, Clone:3C2B8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HepG2 using CL488-66832

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NRF1/nuclear respiratory factor 1 (CL488-66832, Clone:3C2B8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66832 bindet in IF, FC (Intra) NRF1/nuclear respiratory factor 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NRF1/nuclear respiratory factor 1 fusion protein Ag28149
• Vollständiger Name: nuclear respiratory factor 1
• Berechnetes Molekulargewicht: 522 aa, 56 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC016925
• Gene symbol: NRF1
• Gene ID (NCBI): 4899
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011