NRF2, NFE2L2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NRF2, NFE2L2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

104

CloneNo.

1E9E3

Rezensionen (1)

Kat-Nr. : 66504-1-Ig

Synonyme

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2, NRF2, NFE2L2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66504-1-Ig

  Untreated and MG132 treated Hela cells were subjected to SDS PAGE followed by western blot with 66504-1-Ig (NRF2, NFE2L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 13 publications below
• WB: See 96 publications below
• IHC: See 8 publications below
• IF: See 20 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

66504-1-Ig bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA NRF2, NFE2L2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NRF2, NFE2L2 fusion protein Ag9469
• Vollständiger Name: nuclear factor (erythroid-derived 2)-like 2
• Berechnetes Molekulargewicht: 605 aa, 68 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC011558
• Gene symbol: NFE2L2
• Gene ID (NCBI): 4780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Publikationen

Species	Application	Title
human	WB,IHC,IP	J Clin Invest; FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway; Authors - Yunjiang Zhou
mouse	CoIP	Nat Commun; Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection.; Authors - Yao Wei
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu
human	WB	Redox Biol; Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.; Authors - Shumin Ouyang
mouse,human	WB,IF	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human	WB,IHC,IF	Phytomedicine; Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib; Authors - Chen Yang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In tissues, we observed bands at varying positions, but in cancer cell lines, the bands appeared significantly clearer. The optimal concentration was found to be 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines

NRF2, NFE2L2 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:66504-1-Ig)