GABPA Polyklonaler Antikörper

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• KD/KO Validated

GABPA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 21542-1-AP

Synonyme

NFT2, GABP subunit alpha, GA-binding protein alpha chain, GA binding protein alpha chain, E4TF1A

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  WB analysis using 21542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21542-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21542-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 21542-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21542-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21542-1-AP

  IP result of anti-GABPA (IP:21542-1-AP, 4ug; Detection:21542-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 21542-1-AP

  IP result of anti-GABPA (IP:21542-1-AP, 4ug; Detection:21542-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human breast cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21542-1-AP (GABPA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21542-1-AP (GABPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21542-1-AP (GABPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21542-1-AP (GABPA antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HEK-293 using 21542-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GABPA antibody (21542-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, A549-Zellen, K-562-Zellen, MCF-7-Zellen, Maushirngewebe, Mauslebergewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• ChIP: See 1 publications below

Produktinformation

21542-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA GABPA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABPA fusion protein Ag16191
• Vollständiger Name: GA binding protein transcription factor, alpha subunit 60kDa
• Berechnetes Molekulargewicht: 454 aa, 51 kDa
• Beobachtetes Molekulargewicht: 56-60 kDa
• GenBank-Zugangsnummer: BC035031
• Gene symbol: GABPA
• Gene ID (NCBI): 2551
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Publikationen

Species	Application	Title
human	WB	Cell Stem Cell; PSPC1 exerts an oncogenic role in AML by regulating a leukemic transcription program in cooperation with PU.1; Authors - Juyeong Hong
		Hepatology; High mobility group protein B1 controls liver cancer initiation through yes-associated protein -dependent aerobic glycolysis.; Authors - Ruochan Chen
human, mouse	WB,IHC,IF	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H ; KD Validated
mouse	WB	Cardiovasc Res; Sex differences in exercise-induced physiological myocardial hypertrophy are modulated by oestrogen receptor beta.; Authors - Elke Dworatzek
mouse	WB	Stem Cells; MZF1 and GABP Cooperate with Sox2 in Regulating the Expression of YAP1 in Cancer Cells.; Authors - Narendra Kumar Verma ; KD Validated
human	WB	Biology (Basel); TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.; Authors - Yanbing Wang