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NRF2, NFE2L2 Monoklonaler Antikörper

NRF2, NFE2L2 Monoklonal Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E9E3

Kat-Nr. : 66504-1-PBS

Synonyme

NFE2L2, NRF2, 1E9E3, HEBP1, NF-E2-related factor 2

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  • Western Blot (WB) analysis of HeLa cells using NRF2, NFE2L2 Monoclonal antibody (66504-1-Ig)

    WB analysis of HeLa using 66504-1-Ig (same clone as 66504-1-PBS)

    Untreated and MG132 treated Hela cells were subjected to SDS PAGE followed by western blot with 66504-1-Ig (NRF2, NFE2L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66504-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50316-2

    Cytometric bead array standard curve of MP50316-2

    Cytometric bead array standard curve of MP50316-2, NRF2, NFE2L2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66504-2-PBS. Detection antibody: 66504-1-PBS. Standard:Ag9469. Range: 1.563-100 ng/mL.

Geprüfte Anwendungen

Produktinformation

66504-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA NRF2, NFE2L2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen NRF2, NFE2L2 fusion protein Ag9469
Vollständiger Name nuclear factor (erythroid-derived 2)-like 2
Berechnetes Molekulargewicht 605 aa, 68 kDa
Beobachtetes Molekulargewicht 110 kDa
GenBank-ZugangsnummerBC011558
Gene symbol NRF2
Gene ID (NCBI) 4780
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).