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  • Featured Product
  • KD/KO Validated

NRF2, NFE2L2 Rekombinanter Antikörper

NRF2, NFE2L2 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1I21

Kat-Nr. : CL594-80593

Synonyme

NFE2L2, NRF2, HEBP1, NF-E2-related factor 2, NFE2-related factor 2

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated NRF2, NFE2L2 Recombinant m (CL594-80593)

    IF Staining of HepG2 using CL594-80593

    Immunofluorescent analysis of (4% PFA) fixed MG132 treated HepG2 cells using CoraLite®594 NRF2, NFE2L2 antibody (CL594-80593, Clone: 1I21 ) at dilution of 1:200, CL488-phalloidin (green).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCMG132 treated HepG2 cells

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-80593 bindet in IF/ICC NRF2, NFE2L2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NRF2, NFE2L2 fusion protein Ag9489
Vollständiger Name nuclear factor (erythroid-derived 2)-like 2
Berechnetes Molekulargewicht 605 aa, 68 kDa
Beobachtetes Molekulargewicht110 kDa
GenBank-ZugangsnummerBC011558
Gene symbol NRF2
Gene ID (NCBI) 4780
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).