NRG1, isoform SMDF Monoklonaler Antikörper

NRG1, isoform SMDF Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C6D12

Kat-Nr. : 66492-1-PBS

Synonyme

NRG1, NRG1A, 2C6D12, ARIA, GGF

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  WB analysis using 66492-1-Ig (same clone as 66492-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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  WB analysis of pig cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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  WB analysis of rat cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66492-1-Ig (same clone as 66492-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66492-1-Ig (same clone as 66492-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (66492-1-Ig, Clone: 2C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66492-1-PBS bindet in WB, IF/ICC, Indirect ELISA NRG1, isoform SMDF und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NRG1, isoform SMDF fusion protein Ag0803
• Vollständiger Name: neuregulin 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC007675
• Gene symbol: NRG1
• Gene ID (NCBI): 3084
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.