NRG1, isoform SMDF Rekombinanter Antikörper

NRG1, isoform SMDF Rekombinant Antikörper für IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240184G2

Kat-Nr. : 83323-6-PBS

Synonyme

NRG1, NRG1A, 240184G2, ARIA, GGF

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  IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 83323-6-RR (same clone as 83323-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 83323-6-RR (same clone as 83323-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83323-6-RR (same clone as 83323-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83323-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83323-6-RR against Human NRG1, isoform SMDF were performed. The affinity constant is 1.64 nM.

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Geprüfte Anwendungen

Produktinformation

83323-6-PBS bindet in IHC, IF/ICC, IF-P, ELISA NRG1, isoform SMDF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NRG1, isoform SMDF fusion protein Ag0803
• Vollständiger Name: neuregulin 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC007675
• Gene symbol: NRG1
• Gene ID (NCBI): 3084
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform SMDF.