Neuropilin 2 Rekombinanter Antikörper

Neuropilin 2 Rekombinant Antikörper für WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250688C6

Kat-Nr. : 86126-3-PBS

Synonyme

NRP2, Neuropilin-2

---

• 

  WB analysis of U-87 MG using 86126-3-RR (same clone as 86126-3-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 86126-3-RR (NRP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86126-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02281-2

  Sandwich ELISA standard curve of MP02281-2, Human Neuropilin 2 Recombinant Matched Antibody Pair - PBS only. 86126-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3125. 86126-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

• 

  Sample test of MP02281-2

  Serum of six individual healthy human donors was measured. The human Neuropilin 2 concentration of detected samples was determined to be 28.25 ng/mL with a range of 13.50 - 41.26 ng/mL.

• 

  IF Staining of U-87 MG using 86126-3-RR (same clone as 86126-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using Neuropilin 2 antibody (86126-3-RR, Clone: 250688C6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86126-3-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

86126-3-PBS bindet in WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Neuropilin 2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Neuropilin 2 fusion protein Eg3125
• Vollständiger Name: neuropilin 2
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: NM_201266.1
• Gene symbol: Neuropilin 2
• Gene ID (NCBI): 8828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuropilin 2 (NRP2) is a single-chain transmembrane glycoprotein that acts as a co-receptor of VEGF. NRP2 is mainly expressed in lymphatic and venous endothelial cells, and it is associated with the occurrence and development of several types of cancer. NRP2 is known to play a role in cardiovascular development, axon guidance, tumorigenesis, inflammation and cardiovascular disease.