NSD3 Rekombinanter Antikörper

NSD3 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230499C5

Kat-Nr. : 83116-1-RR

Synonyme

WHSC1L1, DC28, EC:2.1.1.370, Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3

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  IHC staining of human lung squamous cell carcinoma using 83116-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83116-1-RR (NSD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 83116-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NSD3 antibody (83116-1-RR, Clone: 230499C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  FC experiment of HeLa using 83116-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human NSD3 (83116-1-RR, Clone:230499C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83116-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83116-1-RR against Human NSD3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: human lung squamous cell carcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83116-1-RR bindet in IHC, IF/ICC, FC (Intra), ELISA NSD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NSD3 fusion protein Ag1894
• Vollständiger Name: Wolf-Hirschhorn syndrome candidate 1-like 1
• Berechnetes Molekulargewicht: 1437 aa, 162 kDa
• GenBank-Zugangsnummer: BC012059
• Gene symbol: WHSC1L1
• Gene ID (NCBI): 54904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404).