NSE Rekombinanter Antikörper

NSE Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250903A11

Kat-Nr. : 86332-1-PBS

Synonyme

ENO2, 2-phospho-D-glycerate hydro-lyase, EC:4.2.1.11, Enolase 2, Gamma enolase

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  WB analysis using 86332-1-RR (same clone as 86332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86332-1-RR (ENO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86332-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86332-1-RR (same clone as 86332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86332-1-RR (ENO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86332-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86332-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86332-1-RR against Human NSE were performed. The affinity constant is 1.16 nM.

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Geprüfte Anwendungen

Produktinformation

86332-1-PBS bindet in WB, Indirect ELISA NSE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NSE fusion protein Ag0196
• Vollständiger Name: enolase 2 (gamma, neuronal)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC002745
• Gene symbol: NSE
• Gene ID (NCBI): 2026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.