NSE Rekombinanter Antikörper

NSE Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250903A11

Kat-Nr. : 86332-1-RR

Synonyme

ENO2, 2-phospho-D-glycerate hydro-lyase, EC:4.2.1.11, Enolase 2, Gamma enolase

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  WB analysis using 86332-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86332-1-RR (ENO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86332-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86332-1-RR (ENO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86332-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86332-1-RR against Human NSE were performed. The affinity constant is 1.16 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, fetales humanes Hirngewebe, HT-29-Zellen, Maushirngewebe, Rattenhirngewebe, SW480-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86332-1-RR bindet in WB, ELISA NSE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NSE fusion protein Ag0196
• Vollständiger Name: enolase 2 (gamma, neuronal)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC002745
• Gene symbol: NSE
• Gene ID (NCBI): 2026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.