NSF Monoklonaler Antikörper

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NSF Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B6H6

Kat-Nr. : 68061-1-PBS

Synonyme

2B6H6, EC:3.6.4.6, NEM-sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, SKD2

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  WB analysis using 68061-1-Ig (same clone as 68061-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 68061-1-Ig (same clone as 68061-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 68061-1-Ig (same clone as 68061-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68061-1-Ig (same clone as 68061-1-PBS)

  WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68061-1-Ig (same clone as 68061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68061-1-Ig (NSF antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 68061-1-Ig (same clone as 68061-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68061-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA NSF und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NSF fusion protein Ag15422
• Vollständiger Name: N-ethylmaleimide-sensitive factor
• Berechnetes Molekulargewicht: 744 aa, 83 kDa
• Beobachtetes Molekulargewicht: 70-83 kDa
• GenBank-Zugangsnummer: BC030613
• Gene symbol: NSF
• Gene ID (NCBI): 4905
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.