NSFL1C Monoklonaler Antikörper

NSFL1C Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F12E12

Kat-Nr. : 68182-1-PBS

Synonyme

1F12E12, NSFL1 cofactor p47, p97 cofactor p47, UBX domain-containing protein 2C, UBXN2C

---

• 

  WB analysis using 68182-1-Ig (same clone as 68182-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68182-1-Ig (same clone as 68182-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68182-1-Ig (same clone as 68182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68182-1-Ig (same clone as 68182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 68182-1-Ig (same clone as 68182-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSFL1C antibody (68182-1-Ig, Clone: 1F12E12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68182-1-PBS bindet in WB, IHC, IF/ICC, ELISA NSFL1C und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NSFL1C fusion protein Ag8040
• Vollständiger Name: NSFL1 (p97) cofactor (p47)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC002801
• Gene symbol: NSFL1C
• Gene ID (NCBI): 55968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.