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NSMCE2 Rekombinanter Antikörper

NSMCE2 Rekombinant Antikörper für WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242862D8

Kat-Nr. : 85188-4-PBS

Synonyme

EC:2.3.2.-, E3 SUMO-protein transferase NSE2, E3 SUMO-protein ligase NSE2, E3 SUMO protein ligase NSE2, C8orf36

Filter:
  • Western Blot (WB) analysis of various lysates using NSMCE2 Recombinant antibody (85188-4-RR)

    WB analysis using 85188-4-RR (same clone as 85188-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85188-4-RR (NSMCE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85188-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01897-2

    Sandwich ELISA standard curve of MP01897-2

    Sandwich ELISA standard curve of MP01897-2, Human NSMCE2 Recombinant Matched Antibody Pair - PBS only. 85188-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4545. 85188-5-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01897-2

    Sample test of MP01897-2

    The mean NSMCE2 concentration was determined to be 137.24 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load, 371.47 ng/mL in Jurkat cell extract based on a 1.90 mg/mL extract load and 481.66 ng/mL in U2OS cell extract based on a 3.50 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NSMCE2 Recombinant antibody (85188-4-RR)

    IF Staining of U2OS using 85188-4-RR (same clone as 85188-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NSMCE2 antibody (85188-4-RR, Clone: 242862D8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85188-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85188-4-RR

    Affinity and Kinetic Characterization of 85188-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85188-4-RR against Human NSMCE2 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85188-4-PBS bindet in WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test NSMCE2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NSMCE2 fusion protein Ag4545
Vollständiger Name non-SMC element 2, MMS21 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 247 aa, 28 kDa
Beobachtetes Molekulargewicht28-32 kDa
GenBank-ZugangsnummerBC032797
Gene symbol NSMCE2
Gene ID (NCBI) 286053
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSMCE2 (E3 SUMO-protein ligase NSE2) has a C-terminal Siz/PIAS-RING (SP-RING) domain with E3 SUMO ligase activity (PMID: 15601841). NSMCE2, an essential SUMO ligase of the SMC5/6 complex suppresses cancer and aging in mice (PMID: 26443207).