NSUN2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NSUN2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(88)

Rezensionen (1)

Kat-Nr. : 20854-1-AP

Synonyme

SAKI, RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203

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  WB analysis of SU-DHL-4 using 20854-1-AP

  WB result of NSUN2 antibody (20854-1-AP; 1:40000; room temperature for 1.5 hours) with wild-type and NSUN2 knockout SU-DHL-4 cells.

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  WB analysis using 20854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20854-1-AP

  WB result of NSUN2 antibody (20854-1-AP; 1:60000; room temperature for 1.5 hours) with sh-Control and sh-NSUN2 transfected HepG2 cells.

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  IP experiment of HeLa using 20854-1-AP

  IP result of anti-NSUN2 (IP:20854-1-AP, 4ug; Detection:20854-1-AP 1:1000) with HeLa cells lysate 4000ug.

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  IHC staining of human breast cancer using 20854-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20854-1-AP (NSUN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20854-1-AP (NSUN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 20854-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20854-1-AP (NSUN2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of A549 using 20854-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 20854-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 20854-1-AP

  Immunofluorescent analysis of HepG2 cells, using NSUN2 antibody 20854-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 20854-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NSUN2 (20854-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:750-1:3000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 34 publications below
• WB: See 79 publications below
• IHC: See 23 publications below
• IF: See 14 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

20854-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA NSUN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NSUN2 fusion protein Ag14791
• Vollständiger Name: NOL1/NOP2/Sun domain family, member 2
• Berechnetes Molekulargewicht: 767 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC001041
• Gene symbol: NSUN2
• Gene ID (NCBI): 54888
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

Publikationen

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis; Authors - Sunyang Ying
human	WB	Nat Biotechnol; Identification of direct targets and modified bases of RNA cytosine methyltransferases.; Authors - Khoddami Vahid V
mouse	WB	Cell; The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.; Authors - Deniz Simsek
human	WB,IHC	Nat Cell Biol; 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.; Authors - Xin Chen
human	WB	Nat Protoc; Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.; Authors - Vahid Khoddami
human	WB	Mol Cell; Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach; Authors - Liang Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah (Verified Customer) (01-05-2021)

Works perfectly for WB. Tested on HeLa cells and HEK293T.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa and HEL293T cells