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NSUN2 Rekombinanter Antikörper

NSUN2 Rekombinant Antikörper für FC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230186A6

Kat-Nr. : 82894-1-RR

Synonyme

FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4

Galerie der Validierungsdaten

Filter:
  • Flow cytometry (FC) experiment of HeLa cells using NSUN2 Recombinant antibody (82894-1-RR)

    FC experiment of HeLa using 82894-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human NSUN2 (82894-1-RR, Clone:230186A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

82894-1-RR bindet in FC, ELISA NSUN2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NSUN2 fusion protein Ag14791
Vollständiger Name NOL1/NOP2/Sun domain family, member 2
Berechnetes Molekulargewicht 767 aa, 86 kDa
GenBank-ZugangsnummerBC001041
Gene symbol NSUN2
Gene ID (NCBI) 54888
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.