NSUN2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

NSUN2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230186G2

Kat-Nr. : 82894-2-RR

Synonyme

RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-

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  WB analysis of HeLa using 82894-2-RR

  WB result of NSUN2 antibody (82894-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN2 transfected HeLa cells.

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  WB analysis using 82894-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82894-2-RR (NSUN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 82894-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-2-RR, Clone: 230186G2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 82894-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NSUN2 antibody (82894-2-RR, Clone: 230186G2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of HeLa using 82894-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NSUN2 Recombinant antibody (82894-2-RR, Clone:230186G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82894-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82894-2-RR against Human NSUN2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-1080.Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:150-1:600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82894-2-RR bindet in WB, IF/ICC, ELISA NSUN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NSUN2 fusion protein Ag14791
• Vollständiger Name: NOL1/NOP2/Sun domain family, member 2
• Berechnetes Molekulargewicht: 767 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC001041
• Gene symbol: NSUN2
• Gene ID (NCBI): 54888
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.