NSUN2 Rekombinanter Antikörper

NSUN2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230186H8

Kat-Nr. : 82894-7-PBS

Synonyme

RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-

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  WB analysis using 82894-7-RR (same clone as 82894-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82894-7-RR (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82894-7-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82894-7-RR (same clone as 82894-7-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82894-7-RR (NSUN2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82894-7-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82894-7-RR (same clone as 82894-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-7-RR, Clone: 230186H8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).CL594-Phalloidin (red). This data was developed using the same antibody clone with 82894-7-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82894-7-RR (same clone as 82894-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NSUN2 Recombinant antibody (82894-7-RR, Clone:230186H8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82894-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82894-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82894-7-RR against Human NSUN2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82894-7-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA NSUN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NSUN2 fusion protein Ag14791
• Vollständiger Name: NOL1/NOP2/Sun domain family, member 2
• Berechnetes Molekulargewicht: 767 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC001041
• Gene symbol: NSUN2
• Gene ID (NCBI): 54888
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.