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NSUN4 Polyklonaler Antikörper

NSUN4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29786-1-PBS

Synonyme

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4

Filter:
  • Western Blot (WB) analysis of various lysates using NSUN4 Polyclonal antibody (29786-1-AP)

    WB analysis using 29786-1-AP (same clone as 29786-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29786-1-AP (NSUN4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NSUN4 Polyclonal antibody (29786-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 29786-1-AP (same clone as 29786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29786-1-AP (NSUN4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29786-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29786-1-PBS bindet in WB, IHC, Indirect ELISA NSUN4 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NSUN4 fusion protein Ag31821
Vollständiger Name NOL1/NOP2/Sun domain family, member 4
Berechnetes Molekulargewicht 384 aa, 43 kDa
Beobachtetes Molekulargewicht40 kDa
GenBank-ZugangsnummerBC014441
Gene symbol NSUN4
Gene ID (NCBI) 387338
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly. The calculated MW of NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.(PMID:22949673, 24516400)