NSUN5 Rekombinanter Antikörper

NSUN5 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242168D5

Kat-Nr. : 84768-2-PBS

Synonyme

NOL1-related protein, NOL1R, NOL1, EC:2.1.1.-, 28S rRNA (cytosine-C(5))-methyltransferase

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  WB analysis using 84768-2-RR (same clone as 84768-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84768-2-RR (NSUN5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84768-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84768-2-RR (same clone as 84768-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84768-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84768-2-RR (same clone as 84768-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84768-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01548-1

  Cytometric bead array standard curve of MP01548-1, NSUN5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84768-1-PBS. Detection antibody: 84768-2-PBS. Standard: Ag7711. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84768-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84768-2-RR against Human NSUN5 were performed. The affinity constant is 0.69 nM.

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Geprüfte Anwendungen

Produktinformation

84768-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA NSUN5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NSUN5 fusion protein Ag7711
• Vollständiger Name: NOL1/NOP2/Sun domain family, member 5
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47~51 kDa
• GenBank-Zugangsnummer: BC008084
• Gene symbol: NSUN5
• Gene ID (NCBI): 55695
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSUN5 is a conserved RNA methyltransferase belonging to the Nop2/SUN domain family, which is highly conserved from archaea to eukaryotes and has close contact with RNA methylation because of their S-adenosyl methionine binding-domain. NSUN5 was a promoter in the progression of CRC mainly through cell cycle regulation (PMID: 32774740). Loss of NSUN5 induces growth phenotypes in mice and different mammalian cells, which is likely connected to the requirement of NSUN5 for ribosomal RNA modification and normal global protein translation, especially in highly proliferative cells (PMID: 31722427). Western blot analysis detected NSUN5 at an apparent molecular mass of 47 kDa.