NSUN7 Rekombinanter Antikörper

NSUN7 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242358B7

Kat-Nr. : 84795-1-PBS

Synonyme

242358B7, FLJ14001

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  WB analysis using 84795-1-RR (same clone as 84795-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84795-1-RR (NSUN7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84795-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 84795-1-RR (same clone as 84795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84795-1-RR (NSUN7 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84795-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01594-1

  Cytometric bead array standard curve of MP01594-1, NSUN7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84795-1-PBS. Detection antibody: 84795-2-PBS. Standard: Ag11650. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84795-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84795-1-RR against Human NSUN7 were performed. The affinity constant is 3.58 nM.

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Geprüfte Anwendungen

Produktinformation

84795-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA NSUN7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NSUN7 fusion protein Ag11650
• Vollständiger Name: NOL1/NOP2/Sun domain family, member 7
• Berechnetes Molekulargewicht: 718 aa, 81 kDa
• Beobachtetes Molekulargewicht: 81~90 kDa
• GenBank-Zugangsnummer: BC036568
• Gene symbol: NSUN7
• Gene ID (NCBI): 79730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).