NT5C2 Polyklonaler Antikörper

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• KD/KO Validated

NT5C2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15223-1-AP

Synonyme

Cytosolic IMP/GMP-specific 5'-nucleotidase, Cytosolic 5'-nucleotidase II, Cytosolic 5 nucleotidase II, cN-II, cN II

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  WB analysis using 15223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15223-1-AP

  WB result of NT5C2 antibody (15223-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5C2 transfected A549 cells.

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  WB analysis of human placenta using 15223-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 15223-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NT5C2 antibody (15223-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, humanes Plazenta-Gewebe, Mauslebergewebe, NCI-H1299-Zellen, NCL-H1299-Zellen, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

15223-1-AP bindet in WB, IHC, IF/ICC, ELISA NT5C2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NT5C2 fusion protein Ag7438
• Vollständiger Name: 5'-nucleotidase, cytosolic II
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC001595
• Gene symbol: NT5C2
• Gene ID (NCBI): 22978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	BMC Med Genomics; Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.; Authors - Xing-Cheng Zhao
mouse	WB,IHC	Cell Death Dis; Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation; Authors - Mei-Xin Li