NT5E/CD73 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NT5E/CD73 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

40

Rezensionen (2)

Kat-Nr. : 12231-1-AP

Synonyme

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD 73, CD73, E5NT, Ecto 5 nucleotidase, ecto-5'-NT, Ecto-5'-nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E/CD73, NTE

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Galerie der Validierungsdaten

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  WB analysis using 12231-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12231-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 12231-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12231-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12231-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 12231-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using NT5E/CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 12231-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using NT5E/CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NCCIT using 12231-1-AP

  1X10^6 NCCIT cell were stained with 0.2ug NT5E/CD73 antibody (12231-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HT-29-Zellen, HuH-7-Zellen, humanes Milzgewebe, Jurkat-Zellen, K-562-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Magengewebe, humanes Magenkrebsgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in FC: NCCIT cell

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 20 publications below
• IHC: See 14 publications below
• IF: See 13 publications below
• ELISA: See 1 publications below
• FC: See 5 publications below

Produktinformation

12231-1-AP bindet in WB, IHC, IF, FC, ELISA NT5E/CD73 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NT5E/CD73 fusion protein Ag2869
• Vollständiger Name: 5'-nucleotidase, ecto (CD73)
• Berechnetes Molekulargewicht: 29 kDa, 63 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC015940
• Gene symbol: NT5E
• Gene ID (NCBI): 4907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa,64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries (CALJA).

Publikationen

Species	Application	Title
human,mouse	IHC,WB,IF	Int J Biol Sci; CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence; Authors - Zhenni Liu
human	WB,IHC	Br J Cancer; Norepinephrine inhibits CD8+ T-cell infiltration and function, inducing anti-PD-1 mAb resistance in lung adenocarcinoma; Authors - Qishun Geng
mouse	IF	Theranostics; GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activity.; Authors - Shuyu Guo
	IHC	Theranostics; A sericin/ graphene oxide composite scaffold as a biomimetic extracellular matrix for structural and functional repair of calvarial bone.; Authors - Chao Qi
human	WB	Biochim Biophys Acta Mol Basis Dis; Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages; Authors - Ling Zhou
rat	FC	J Cell Physiol; Mesenchymal stem cells overexpressing heme oxygenase-1 ameliorate lipopolysaccharide-induced acute lung injury in rats.; Authors - Xuxin Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

good product for ICC

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: ROs

FH

Neta (Verified Customer) (07-28-2022)

staining did not work

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse small intestine, fixed frozen