NT5E Rekombinanter Antikörper

NT5E Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251250E3

Kat-Nr. : 86512-1-PBS

Synonyme

CD73, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

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  Sandwich ELISA sample test of MP02481-1

  The mean CD73 concentration was determined to be 67.84 ng/mL in U-87 MG cell extract based on a 1.5 mg/mL extract load and 125.52 ng/mL in PC-3 cell extract based on a 1.1 mg/mL extract load.

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  Sandwich ELISA sample test of MP02481-1

  Plasma of six individual healthy human donors was measured. The human CD73 concentration of detected samples was determined to be 15.14 ng/mL with a range of 8.55 - 28.73 ng/mL.

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  Sandwich ELISA standard curve of MP02481-1

  Sandwich ELISA standard curve of MP02481-1, Human CD73 Recombinant Matched Antibody Pair - PBS only. 86512-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2974. 86512-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Cytometric bead array sample test of MP02481-1

  The mean CD73 concentration was determined to be 895.3 ng/mL in U-87 MG cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP02481-1

  Cytometric bead array standard curve of MP02481-1, CD73 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86512-2-PBS. Detection antibody: 86512-1-PBS. Standard: Eg2974. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86512-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 86512-1-PBS against Human NT5E was performed. The affinity constant is 1.63 nM.

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Geprüfte Anwendungen

Produktinformation

86512-1-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA NT5E und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NT5E fusion protein Eg2974
• Vollständiger Name: 5'-nucleotidase, ecto (CD73)
• Berechnetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: NM_002526.3
• Gene symbol: CD73
• Gene ID (NCBI): 4907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.