CD73 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CD73 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(2)

CloneNo.

1G5G8

Kat-Nr. : CL488-67789

Synonyme

NT5E, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73), 5 nucleotidase

---

• 

  IF Staining of human appendicitis using CL488-67789

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite® Plus 488 NT5E/CD73 antibody (CL488-67789, Clone: 1G5G8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human appendicitis using CL488-67789

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite® Plus 488 NT5E/CD73 antibody (CL488-67789, Clone: 1G5G8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using CL488-67789

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 NT5E/CD73 antibody (CL488-67789, Clone: 1G5G8 ) at dilution of 1:200.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Appendizitis-Gewebe, humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

CL488-67789 bindet in IF-P CD73 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD73 fusion protein Ag30616
• Vollständiger Name: 5'-nucleotidase, ecto (CD73)
• Berechnetes Molekulargewicht: 29 kDa, 63 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC015940
• Gene symbol: CD73
• Gene ID (NCBI): 4907
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Publikationen

Species	Application	Title
human	IF	Front Immunol; NT5E upregulation in head and neck squamous cell carcinoma: A novel biomarker on cancer-associated fibroblasts for predicting immunosuppressive tumor microenvironment; Authors - Xue-min Chen
	IF	Front Pharmacol; Pan-cancer analysis identifies NT5E as a novel prognostic biomarker on cancer-associated fibroblasts associated with unique tumor microenvironment; Authors - Xin-Miao Xue