NT5M Polyklonaler Antikörper

NT5M Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20765-1-AP

Synonyme

5'(3')-deoxyribonucleotidase, mitochondrial, 5',3'-nucleotidase, mitochondrial, Deoxy-5'-nucleotidase 2, dNT 2, DNT2

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  WB analysis using 20765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 20765-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 20765-1-AP

  IP result of anti-NT5M (IP:20765-1-AP, 4ug; Detection:20765-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20765-1-AP bindet in WB, IP, ELISA NT5M und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NT5M fusion protein Ag14743
• Vollständiger Name: 5',3'-nucleotidase, mitochondrial
• Berechnetes Molekulargewicht: 228 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC035838
• Gene symbol: NT5M
• Gene ID (NCBI): 56953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NT5M is a mitochondrial 5′(3′)-deoxyribonucleotidase that plays a crucial role in maintaining balanced deoxynucleotide pools essential for mitochondrial DNA synthesis. It dephosphorylates specific nucleoside monophosphates, particularly dUMP and dTMP, thereby preventing the accumulation of toxic dTTP. NT5M is predominantly localized in mitochondria and is highly expressed in tissues such as the heart, brain, and skeletal muscle.