NTB-A/SLAMF6 Rekombinanter Antikörper

NTB-A/SLAMF6 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241384G5

Kat-Nr. : 84211-4-RR

Synonyme

SLAMF6, Ly108, KALIb, KALI, CD352

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  WB analysis using 84211-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84211-4-RR (NTB-A/SLAMF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Ramos using 84211-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using NTB-A/SLAMF6 antibody (84211-4-RR, Clone: 241384G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84211-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84211-4-RR against Human NTB-A/SLAMF6 were performed. The affinity constant is 2.73 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84211-4-RR bindet in WB, IF/ICC, ELISA NTB-A/SLAMF6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: SLAM family member 6
• Berechnetes Molekulargewicht: 37kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_001184714.2
• Gene symbol: SLAMF6
• Gene ID (NCBI): 114836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signaling lymphocyte activation molecule 6 (SLAMF6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules (PMID:31199820). SLAMF6 is a type I transmembrane protein with two extracellular immunoglobins (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs, one of which is immunoreceptor tyrosine-based switch motif (PMID:31315913). SLAMF6 is expressed on a wide variety of immune cells including T cells (also TFH), B cells, NK cells (expressed in humans only), double positive thymocytes, eosinophils, and neutrophils (mouse only) (PMID:11862385).