Neurotrophin 3 Polyklonaler Antikörper

Neurotrophin 3 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 18084-1-AP

Synonyme

NTF3, HDNF, Neurotrophin-3, NT 3, NT3

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  WB analysis of human placenta using 18084-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18084-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 18084-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Produktinformation

18084-1-AP bindet in WB, IHC, IF/ICC, ELISA Neurotrophin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neurotrophin 3 fusion protein Ag12798
• Vollständiger Name: neurotrophin 3
• Berechnetes Molekulargewicht: 257 aa, 29 kDa
• GenBank-Zugangsnummer: BC069773
• Gene symbol: Neurotrophin 3
• Gene ID (NCBI): 4908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neurotrophin-3 (NT-3) is a member of the neurotrophin family, which are growth factor proteins that play essential roles in the development and maintenance of the nervous system. NT-3 is synthesized by fibroblasts and has been extensively studied for its role in the development of neurons and neural crest-derived cells, such as melanocytes. It can bind to each of the Trk receptors (Trk-A, Trk-B, and Trk-C), but it primarily functions by binding to Trk-C, modulating intracellular signal transduction through the MAPK and PI3K-Akt pathways, which regulate melanocyte differentiation and survival.

Publikationen

Species	Application	Title
mouse	IHC	ACS Appl Mater Interfaces; Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.; Authors - Jiaojiao Zhu
human	WB	Stem Cell Res Ther; NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.; Authors - Ke Wu
human	IHC	Invest Ophthalmol Vis Sci; Alteration of Neurotrophic Factors and Innervation in Extraocular Muscles of Individuals With Concomitant Esotropia; Authors - Jie Hao
	WB	Front Pharmacol; Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.; Authors - Bin Yu
	IHC	Front Genet; EDN1 and NTF3 in keloid pathogenesis: computational and experimental evidence as novel diagnostic biomarkers for fibrosis and inflammation; Authors - Hui Gong
mouse	WB	Brain Res Bull; PPARα contributes to the therapeutic effect of hydrogen gas against sepsis-associated encephalopathy with the regulation to the CREB-BDNF signaling pathway and hippocampal neuron plasticity-related gene expression.; Authors - Yuanyuan Bai