Neurotrophin 3 Polyklonaler Antikörper

Neurotrophin 3 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 18084-1-AP

Synonyme

HDNF, Nerve growth factor 2, Neurotrophic factor, neurotrophin 3, NGF 2, NGF2, NT 3, NT3, NTF3

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 18084-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18084-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 18084-1-AP

  IP Result of anti-Neurotrophin 3 (IP:18084-1-AP, 4ug; Detection:18084-1-AP 1:800) with SH-SY5Y cells lysate 1600ug.

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  IF Staining of SH-SY5Y using 18084-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

18084-1-AP bindet in WB, IP, IHC, IF, ELISA Neurotrophin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neurotrophin 3 fusion protein Ag12798
• Vollständiger Name: neurotrophin 3
• Berechnetes Molekulargewicht: 257 aa, 29 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC069773
• Gene symbol: NTF3
• Gene ID (NCBI): 4908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	ACS Appl Mater Interfaces; Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.; Authors - Jiaojiao Zhu
human	WB	Stem Cell Res Ther; NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.; Authors - Ke Wu
	WB	Front Pharmacol; Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.; Authors - Bin Yu
mouse	WB	Brain Res Bull; PPARα contributes to the therapeutic effect of hydrogen gas against sepsis-associated encephalopathy with the regulation to the CREB-BDNF signaling pathway and hippocampal neuron plasticity-related gene expression.; Authors - Yuanyuan Bai
rat	WB,IHC	Neurochem Res; Human Umbilical Cord Mesenchymal Stem Cells Overexpressing Nerve Growth Factor Ameliorate Diabetic Cystopathy in Rats.; Authors - Wu WenBo
mouse	IF	Neurosci Lett; Matrine promotes NT3 expression in CNS cells in experimental autoimmune encephalomyelitis.; Authors - Ming-Liang Zhang