Neurotrophin 4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Neurotrophin 4 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12297-1-AP

Synonyme

NTF4, NT-4, NT4, NT 4, Neutrophic factor 4

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  WB analysis using 12297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12297-1-AP (Neurotrophin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 12297-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using Neurotrophin 4 antibody (12297-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 12297-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 12297-1-AP (Neurotrophin 4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maushirngewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe, Rattennierengewebe
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12297-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA Neurotrophin 4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neurotrophin 4 fusion protein Ag2938
• Vollständiger Name: neurotrophin 4
• Berechnetes Molekulargewicht: 210 aa, 22 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC012421
• Gene symbol: Neurotrophin 4
• Gene ID (NCBI): 4909
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925).

Publikationen

Species	Application	Title
human	WB,IHC,IF,CoIP	Int J Oncol; Upregulated NTF4 in colorectal cancer promotes tumor development via regulating autophagy.; Authors - Zhou Yang ; KD Validated
human	WB	Onco Targets Ther; Analysis of risk factors for colon cancer progression.; Authors - Zhou Yang