NTHL1 Polyklonaler Antikörper

NTHL1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 14918-1-AP

Synonyme

NTH1, NTHL1, OCTS3

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  WB analysis using 14918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14918-1-AP (NTHL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 14918-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14918-1-AP (NTHL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 14918-1-AP

  IP result of anti-NTHL1 (IP:14918-1-AP, 4ug; Detection:14918-1-AP 1:500) with Y79 cells lysate 2000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, Y79-Zellen
• Erfolgreiche IP: Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14918-1-AP bindet in WB, IP, ELISA NTHL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NTHL1 fusion protein Ag6734
• Vollständiger Name: nth endonuclease III-like 1 (E. coli)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000391
• Gene symbol: NTHL1
• Gene ID (NCBI): 4913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

Publikationen

Species	Application	Title
human	WB	Nature; Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.; Authors - Shangming Tang
human	WB	Nat Commun; Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC; Authors - Nathan P Ward