TrkB Polyklonaler Antikörper

TrkB Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, Peptide array, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

55

Kat-Nr. : 13129-1-AP

Synonyme

GP145 TrkB, NTRK2, TrkB, TrkB tyrosine kinase

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 13129-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 13129-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 13129-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spinal cord using 13129-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse cerebellum using 13129-1-AP

  IP Result of anti-TrkB (IP:13129-1-AP, 4ug; Detection:13129-1-AP 1:300) with mouse cerebellum tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 13129-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13129-1-AP (TrkB antibody) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 13129-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13129-1-AP (TrkB antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Hausschwein-Cerebellum-Gewebe, humanes Cerebellum-Gewebe, humanes Magengewebe, Maus-Cerebellum-Gewebe, Maus-Rückenmarkgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 51 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Produktinformation

13129-1-AP bindet in WB, Peptide array, IP, IHC, IF, CoIP, ELISA TrkB und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TrkB fusion protein Ag3790
• Vollständiger Name: neurotrophic tyrosine kinase, receptor, type 2
• Berechnetes Molekulargewicht: 822 aa, 92 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa, 140-145 kDa
• GenBank-Zugangsnummer: BC031835
• Gene symbol: NTRK2
• Gene ID (NCBI): 4915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).

Publikationen

Species	Application	Title
rat	WB	Chemosphere; Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.; Authors - Mingdan You
rat	WB	Oxid Med Cell Longev; Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway.; Authors - Yaran Gao
mouse	WB	J Affect Disord; Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel; Authors - Hui Yao
rat	WB	ACS Chem Neurosci; Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine.; Authors - Lian Liu
human	IHC	Am J Cancer Res; LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance.; Authors - Zhilan Li
human	WB,IF	Cell Biosci; A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.; Authors - Juan Yin

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