NUAK2 Polyklonaler Antikörper

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• KD/KO Validated

NUAK2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11592-1-AP

Synonyme

NUAK family SNF1 like kinase 2, NUAK2, SNARK, SNF1/AMP kinase related kinase

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Galerie der Validierungsdaten

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  WB analysis of Y79 using 11592-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11592-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11592-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 11592-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 11592-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 11592-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11592-1-AP (NUAK2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 11592-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11592-1-AP (NUAK2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH3T3 cells using 11592-1-AP

  NIH3T3 cells on cover slips fixed and staining with anti-NUAK2 (11592-1-AP,1:100;Rabbit polyclonal -Red) and anti-pericentrin(centrosome Marker; McAb-Green). The photo shows the centrosome in cell cycle: Interphase, Prophase, Prometaphase, Metaphase, Anaphase and Telophase. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml.(from Dr. Zineldeen Doaa Hussein) .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

11592-1-AP bindet in WB, IHC, IF, ELISA NUAK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUAK2 fusion protein Ag2031
• Vollständiger Name: NUAK family, SNF1-like kinase, 2
• Berechnetes Molekulargewicht: 628 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC017306
• Gene symbol: NUAK2
• Gene ID (NCBI): 81788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUAK2(NUAK family SNF1-like kinase 2) is also named as OMPHK2, SNARK and belongs to the SNF1 subfamily. The function of NUAK2 is partly dependent on anti-apoptotic properties against apoptosis induced by death ligand and NUAK2 also has effects on the migration of cancer cells as speculated from studies on myosin filaments and cytoskeleton organization in normal cells(PMID:21911917). Western blot analysis detected NUAK2 expressed in cancer cell lines of diverse tissue origin(PMID:12409306).

Publikationen

Species	Application	Title
human	WB,IHC	Proc Natl Acad Sci U S A; AMP kinase-related kinase NUAK2 affects tumor growth, migration, and clinical outcome of human melanoma.; Authors - Namiki Takeshi T
human	WB, IF	Cancer Res; NUAK2 amplification coupled with PTEN deficiency promote melanoma development via CDK activation.; Authors - Takeshi Namiki ; KD Validated
human	WB	Diabetologia; Regulation of skeletal muscle sucrose, non-fermenting 1/AMP-activated protein kinase-related kinase (SNARK) by metabolic stress and diabetes.; Authors - Rune A A ; KD Validated
human	WB, IF	Ann Clin Biochem; Sucrose non-fermenting AMPK related kinase/Pentraxin 3 and DNA damage axis: a gateway to cardiovascular disease in systemic lupus erythematosus among Egyptian patients.; Authors - Doaa Hussein Zineldeen