NUCKS1 Polyklonaler Antikörper

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• KD/KO Validated

NUCKS1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12023-2-PBS

Synonyme

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  WB analysis using 12023-2-AP (same clone as 12023-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 12023-2-AP (same clone as 12023-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 12023-2-AP (same clone as 12023-2-PBS)

  IP result of anti-NUCKS1 (IP:12023-2-AP, 4ug; Detection:12023-2-AP 1:600) with HEK-293 cells lysate 960 ug. This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12023-2-AP (same clone as 12023-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 12023-2-AP (same clone as 12023-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 12023-2-AP (same clone as 12023-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12023-2-AP (same clone as 12023-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12023-2-PBS bindet in WB, IHC, IP, Indirect ELISA NUCKS1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUCKS1 fusion protein Ag2652
• Vollständiger Name: nuclear casein kinase and cyclin-dependent kinase substrate 1
• Berechnetes Molekulargewicht: 243 aa, 27 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC000805
• Gene symbol: NUCKS1
• Gene ID (NCBI): 64710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1) is a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated by CDK1 and casein kinase during mitosis of the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Widelyexpressed, with highest levels in thyroid gland, prostate and uterus and in fetal liver, thymus and lung. Two isoforms of NUCKS1 exist due to alternative splicing events.