NUDC Polyklonaler Antikörper

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• KD/KO Validated

NUDC Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10681-1-PBS

Synonyme

MNUDC, HNUDC

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  WB analysis of HepG2 using 10681-1-AP (same clone as 10681-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 10681-1-AP (same clone as 10681-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10681-1-AP (same clone as 10681-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10681-1-AP (same clone as 10681-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 10681-1-AP (same clone as 10681-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10681-1-AP (same clone as 10681-1-PBS)

  IP result of anti-NUDC (IP:10681-1-AP, 4ug; Detection:10681-1-AP 1:1000) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10681-1-AP (same clone as 10681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10681-1-AP (NUDC antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10681-1-AP (same clone as 10681-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDC antibody (10681-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10681-1-AP (same clone as 10681-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 10681-1-AP (same clone as 10681-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10681-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10681-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NUDC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUDC fusion protein Ag0998
• Vollständiger Name: nuclear distribution gene C homolog (A. nidulans)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa
• GenBank-Zugangsnummer: BC006147
• Gene symbol: NUDC
• Gene ID (NCBI): 10726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear distribution protein C (NudC) is a highly conserved dynein/dynactin associated protein implicated in mitosis and cytokinesis. NudC is localized on major mitotic structures, and is involved in regulating spindle formation stability of kinetochore microtubule attachments, and chromosomes congression in early mitosis. NudC also is required for multi-protein transport along axons in neurons.