NUDCD2 Polyklonaler Antikörper

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• KD/KO Validated

NUDCD2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 21205-1-AP

Synonyme

NudCL2, NudC domain-containing protein 2, NudC domain containing 2

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  WB analysis using 21205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21205-1-AP

  WB result of NUDCD2 antibody (21205-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDCD2 transfected HEK-293 cells.

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  WB analysis of human brain using 21205-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21205-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21205-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 21205-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21205-1-AP

  IP result of anti-NUDCD2 (IP:21205-1-AP, 4ug; Detection:21205-1-AP 1:500) with HepG2 cells lysate 1080 ug.

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  IHC staining of human kidney using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21205-1-AP (NUDCD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 21205-1-AP

  Immunofluorescent analysis of HepG2 cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 21205-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21205-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 21205-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 21205-1-AP

  Immunofluorescent analysis of Hela cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, humanes Hirngewebe, humanes Nierengewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21205-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NUDCD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUDCD2 fusion protein Ag15579
• Vollständiger Name: NudC domain containing 2
• Berechnetes Molekulargewicht: 157 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC017934
• Gene symbol: NUDCD2
• Gene ID (NCBI): 134492
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Life Sci; RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells; Authors - Wanzhi Cai