NUDCD2 Rekombinanter Antikörper

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• KD/KO Validated

NUDCD2 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

230513A5

Kat-Nr. : 83217-5-PBS

Synonyme

230513A5, NudC domain containing 2, NudC domain-containing protein 2, NudCL2

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  WB analysis using 83217-5-RR (same clone as 83217-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83217-5-RR (NUDCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83217-5-RR (same clone as 83217-5-PBS)

  WB result of NUDCD2 antibody (83217-5-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDCD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

  IP result of anti-NUDCD2 (IP:83217-5-RR, 4ug; Detection:83217-5-RR 1:4000) with HepG2 cells lysate 1085 ug. This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (83217-5-RR, Clone: 230513A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUDCD2 Recombinant antibody (83217-5-RR, Clone:230513A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83217-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83217-5-RR against Human NUDCD2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83217-5-PBS bindet in WB, IF/ICC, FC (Intra), IP, ELISA NUDCD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NUDCD2 fusion protein Ag15579
• Vollständiger Name: NudC domain containing 2
• Berechnetes Molekulargewicht: 157 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC017934
• Gene symbol: NUDCD2
• Gene ID (NCBI): 134492
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).