MTH1 Polyklonaler Antikörper

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• KD/KO Validated

MTH1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16705-1-AP

Synonyme

NUDT1, 2-hydroxy-dATP diphosphatase, 7,8-dihydro-8-oxoguanine triphosphatase, 8 oxo dGTPase, 8-oxo-dGTPase

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  WB analysis of Jurkat using 16705-1-AP

  WB result of MTH1 antibody (16705-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTH1 transfected Jurkat cells.

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  WB analysis of HepG2 using 16705-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16705-1-AP (MTH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16705-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16705-1-AP (MTH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16705-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16705-1-AP (MTH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16705-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16705-1-AP (MTH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16705-1-AP (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 16705-1-AP

  Immunofluorescent analysis of PC-3 cells using 16705-1-AP (MTH1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, Jurkat-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

16705-1-AP bindet in WB, IHC, IF/ICC, ELISA MTH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTH1 fusion protein Ag10103
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 1
• Berechnetes Molekulargewicht: 179aa,20 kDa; 197aa,23 kDa
• Beobachtetes Molekulargewicht: 18-24 kDa
• GenBank-Zugangsnummer: BC014618
• Gene symbol: MTH1
• Gene ID (NCBI): 4521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Farnesyl phenolic enantiomers as natural MTH1 inhibitors from Ganoderma sinense.; Authors - Ya Gao ; KD Validated
mouse	WB	Colloids Surf B Biointerfaces; Ultrasound-triggered reactive oxygen species effector nanoamplifier for enhanced combination therapy of mutant p53 tumors.; Authors - Xiang Wang
mouse	WB	Photodiagnosis Photodyn Ther; Cepharanthine synergizes with photodynamic therapy for boosting ROS-driven DNA damage and suppressing MTH1 as a potential anti-cancer strategy; Authors - Zi-Yi Yang
human	WB	BMC Gastroenterol; USP9X regulates the proliferation, survival, migration and invasion of gastric cancer cells by stabilizing MTH1; Authors - Wenji Xu