NUDT3/4/10/11 Polyklonaler Antikörper

NUDT3/4/10/11 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17508-1-AP

Synonyme

NUDT10, DIPP3A, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, Diadenosine hexaphosphate hydrolase, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha

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  WB analysis using 17508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17508-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17508-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17508-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17508-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDT3/4/10/11 antibody (17508-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17508-1-AP bindet in WB, IF/ICC, ELISA NUDT3/4/10/11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUDT3/4/10/11 fusion protein Ag11711
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 10
• Berechnetes Molekulargewicht: 164 aa, 19 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC050700
• Gene symbol: NUDT10
• Gene ID (NCBI): 170685
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUDT10 (nucleoside diphosphate linked moiety X-type motif 10), also named as DIPP3A or APS2, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).