NUDT3/4/10/11 Polyklonaler Antikörper

NUDT3/4/10/11 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 10912-1-AP

Synonyme

APS1, ASP1, DIPP 3 beta, DIPP3 beta, DIPP3b, DIPP3beta, hAps1, hDIPP3beta, Nudix motif 11, NUDT11

---

• 

  WB analysis using 10912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10912-1-AP (NUDT11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10912-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10912-1-AP (NUDT11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10912-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10912-1-AP (NUDT11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10912-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

10912-1-AP bindet in WB, IHC, ELISA NUDT3/4/10/11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUDT3/4/10/11 fusion protein Ag1346
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 11
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC009942
• Gene symbol: NUDT11
• Gene ID (NCBI): 55190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUDT11 (nucleoside diphosphate linked moiety X-type motif 11), also named as DIPP3B or APS1, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).

Publikationen

Species	Application	Title
mouse	WB,IHC	Sci Rep; Construction of a diagnostic model utilizing m7G regulatory factors for the characterization of diabetic nephropathy and the immune microenvironment; Authors - Jingying Zhong