NUDT15 Polyklonaler Antikörper

NUDT15 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25914-1-AP

Synonyme

8 oxo dGTPase NUDT15, EC:3.6.1.9, MTH2, MutT homolog 2, Nucleoside diphosphate-linked moiety X motif 15

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  WB analysis using 25914-1-AP

  Jurkat lysates were subjected to SDS PAGE followed by western blot with 25914-1-AP (NUDT15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25914-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25914-1-AP (NUDT15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25914-1-AP bindet in WB, ELISA NUDT15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUDT15 fusion protein Ag23242
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 15
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC133015
• Gene symbol: NUDT15
• Gene ID (NCBI): 55270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUDT15 (nucleoside diphosphate-linked moiety X-type motif 15) is homologues to NUDT1 (MutT homologue 1, MTH1) and thus has previously been referred to as MTH2. NUDT15 is a key enzyme in thiopurine metabolism and tempers the activity of thiopurine drugs by catalyzing the hydrolysis of 6-thio-dGTP and 6-thio-GTP (PMID: 27530327, 32690945). NUDT15 is a 164-amino-acid protein that belongs to the nudix hydrolase enzyme family, whose members are capable of hydrolyzing compounds with the general structure of a nucleoside diphosphate linked to another moiety, X. (PMID: 25108385)