NUDT21 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NUDT21 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C4C8

Kat-Nr. : 66335-1-PBS

Synonyme

Nucleoside diphosphate-linked moiety X motif 21, CPSF5, CPSF25, Cleavage factor Im complex 25 kDa subunit, CFIm25

---

• 

  WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

  WB result of NUDT21 antibody (66335-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDT21 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66335-1-Ig (same clone as 66335-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66335-1-Ig (same clone as 66335-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66335-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NUDT21 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NUDT21 fusion protein Ag12260
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 21
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC001403
• Gene symbol: NUDT21
• Gene ID (NCBI): 11051
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.