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NUDT5 Polyklonaler Antikörper

NUDT5 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27004-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using NUDT5 Polyclonal antibody (27004-1-AP)

    WB analysis using 27004-1-AP (same clone as 27004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27004-1-AP (NUDT5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using NUDT5 Polyclonal antibody (27004-1-AP)

    IHC staining of mouse liver using 27004-1-AP (same clone as 27004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27004-1-AP (NUDT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27004-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUDT5 Polyclonal antibody (27004-1-AP)

    IF Staining of HeLa using 27004-1-AP (same clone as 27004-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT5 antibody (27004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27004-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27004-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NUDT5 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NUDT5 fusion protein Ag25485
Vollständiger Name nudix (nucleoside diphosphate linked moiety X)-type motif 5
Berechnetes Molekulargewicht 24 kDa
Beobachtetes Molekulargewicht 35 kDa
GenBank-ZugangsnummerBC000025
Gene symbol NUDT5
Gene ID (NCBI) 11164
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUDT5 (NUDIX hydrolase type 5), also called NUDIX5, is a member of the nudix hydrolase family which eliminate toxic nucleotide derivatives from the cell. NUDT5 expression can affect chromosome remodeling, involved in cell adhesion, cancer stem cell maintenance and epithelial to mesenchyme transition in breast cancer cells. In addtion, the high expression of NUDT5 is probably involved in the poor prognosis of breast cancer, which could be a prognostic factor and potential target in the diagnosis and treatment of breast cancer (PMID: 33571243). This NUDT5 protein can be detected with a molecular weight of 35 kDa (PMID: 33096144).