NUDT6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NUDT6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 11181-1-AP

Synonyme

gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.-

---

• 

  WB analysis of Jurkat using 11181-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11181-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11181-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11181-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11181-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11181-1-AP

  IP result of anti-NUDT6 (IP:11181-1-AP, 4ug; Detection:11181-1-AP 1:500) with HepG2 cells lysate 4000ug.

• 

  IHC staining of human liver using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11181-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUDT6 antibody (11181-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, humanes Herzgewebe, Rattenherzgewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maushirngewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11181-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NUDT6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUDT6 fusion protein Ag1638
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 6
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC009842
• Gene symbol: NUDT6
• Gene ID (NCBI): 11162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.

Publikationen

Species	Application	Title
rat	WB,IF	J Physiol; FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling; Authors - Burak Uzay
human	WB	Mol Endocrinol; Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.; Authors - MacFarlane Leigh-Ann LA
human	WB	Mol Carcinog; Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression.; Authors - MacFarlane Leigh-Ann LA
mouse	WB	Cancer Sci; PMID: 29532562; Authors - Ji-Chang Wang
rat	WB	Genes (Basel); Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.; Authors - Mark Baguma-Nibasheka ; KD Validated
human	WB	Wound Repair Regen; GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.; Authors - Xinying Wang