NUDT8 Polyklonaler Antikörper

NUDT8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16098-1-AP

Synonyme

EC:3.6.1.-, Mitochondrial coenzyme A diphosphatase NUDT8, Nucleoside diphosphate-linked moiety X motif 8, Nudix motif 8

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  WB analysis using 16098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Milzgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16098-1-AP bindet in WB, IHC, ELISA NUDT8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUDT8 fusion protein Ag8945
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 8
• Berechnetes Molekulargewicht: 236 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC018644
• Gene symbol: NUDT8
• Gene ID (NCBI): 254552
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUDT8 belongs to the Nudix hydrolase family, which is involved in intracellular metabolic processes, especially related to the decomposition and degradation of nucleotides. NUDT8 is a novel CoA diphosphohydrolase that localizes to the mitochondria and is broadly expressed in mitochondria-rich organs such as the heart, kidneys, liver, and brown adipose tissue(PMID: 31004344).