NUFIP2 Polyklonaler Antikörper

NUFIP2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 17752-1-AP

Synonyme

182 FIP, 82 FIP, 82 kDa FMRP-interacting protein, 82-FIP, Cell proliferation-inducing gene 1 protein

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  WB analysis of HeLa using 17752-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17752-1-AP (NUFIP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17752-1-AP

  IP result of anti-NUFIP2 (IP:17752-1-AP, 4ug; Detection:17752-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human cerebellum using 17752-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 17752-1-AP (NUFIP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 17752-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 17752-1-AP (NUFIP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

17752-1-AP bindet in WB, IHC, IF, IP, ELISA NUFIP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUFIP2 fusion protein Ag12094
• Vollständiger Name: nuclear fragile X mental retardation protein interacting protein 2
• Berechnetes Molekulargewicht: 695 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76-82 kDa
• GenBank-Zugangsnummer: BC108307
• Gene symbol: NUFIP2
• Gene ID (NCBI): 57532
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Cell; Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.; Authors - David W Sanders
mouse	IHC	Proc Natl Acad Sci U S A; Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasis; Authors - Austin C Boese
human	WB	J Cell Biol; Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.; Authors - Jonathan Bizarro
		Neuron; Increased G3BP2-Tau interaction in tauopathies is a natural defense against Tau aggregation; Authors - Congwei Wang
		Cell Rep; The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network; Authors - Zhiying Yao
mouse	WB,IF	Neurobiol Dis; ATXN2L primarily interacts with NUFIP2, the absence of ATXN2L results in NUFIP2 depletion, and the ATXN2-polyQ expansion triggers NUFIP2 accumulation; Authors - Jana Key