NUFIP2 Monoklonaler Antikörper

NUFIP2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2A4E3

Rezensionen (1)

Kat-Nr. : 67195-1-Ig

Synonyme

Cell proliferation-inducing gene 1 protein, 82-FIP, 82 kDa FMRP-interacting protein, 82 FIP, 2A4E3

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  WB analysis using 67195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67195-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67195-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67195-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 67195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67195-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUFIP2 antibody (67195-1-Ig, Clone: 2A4E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

67195-1-Ig bindet in WB, IHC, IF/ICC, ELISA NUFIP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NUFIP2 fusion protein Ag29040
• Vollständiger Name: nuclear fragile X mental retardation protein interacting protein 2
• Berechnetes Molekulargewicht: 695 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76 kDa, 82 kDa
• GenBank-Zugangsnummer: BC108307
• Gene symbol: NUFIP2
• Gene ID (NCBI): 57532
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Neurobiol Dis; ATXN2L primarily interacts with NUFIP2, the absence of ATXN2L results in NUFIP2 depletion, and the ATXN2-polyQ expansion triggers NUFIP2 accumulation; Authors - Jana Key

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

An excellent antibody with great sensitivity and specificity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T